A 96-Well Plate Assay for CYP4503A Induction Using Cryopreserved Human Hepatocytes

Short Communication A 96-Well Plate Assay for CYP4503A Induction Using Cryopreserved Human Hepatocytes

A reliable and practical CYP3A induction assay with cryopreserved human hepatocytes in a 96-well format was developed. Various 96-well plates with different basement membrane were evaluated using prototypical inducers, rifampicin, phenytoin, and carbamazepine. Thin-layer (TL) Matrigel was found to yield the highest basal and induced levels of CYP3A activity as determined by testosterone 6 -hydr...

A 96-well plate assay for CYP4503A induction using cryopreserved human hepatocytes.

A reliable and practical CYP3A induction assay with cryopreserved human hepatocytes in a 96-well format was developed. Various 96-well plates with different basement membrane were evaluated using prototypical inducers, rifampicin, phenytoin, and carbamazepine. Thin-layer (TL) Matrigel was found to yield the highest basal and induced levels of CYP3A activity as determined by testosterone 6β-hydr...

A 96-well Plate Assay for CYP3A Induction Using Cryopreserved Human Hepatocytes

A 96-Well Plate Assay for Relative Monoclonal Antibody Titers

LEVEL: INTERMEDIATE M onoclonal antibodies are used to treat a wide range of illnesses such as rheumatoid arthritis, Crohn’s Disease, and follicular non-Hodgkin lymphoma (1). The identification and use of certain growth medium additives and modified culture conditions during cell culture processes that produce MAbs may lower production costs—which could help lower the overall costs of those dru...

Evaluation of antibacterial activity of Australian basidiomycetous macrofungi using a high-throughput 96-well plate assay.

CONTEXT The production of antimicrobial compounds by macrofungi is not unexpected because they have to compete with other organisms for survival in their natural hostile environment. Previous studies have indicated that macrofungi contain secondary metabolites with a range of pharmacological activities including antimicrobial agents. OBJECTIVE To investigate macrofungi for antimicrobial activ...